The GBA3 Polyclonal Antibody (CAB7827) is a valuable tool for researchers studying GBA3, a crucial enzyme involved in lipid metabolism. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the GBA3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in biochemistry and lipid-related research.GBA3, also known as glucosylceramidase, is essential for the breakdown of glycosphingolipids, key components of cell membranes.
Dysregulation of GBA3 activity has been linked to lipid storage disorders and neurodegenerative diseases, highlighting the importance of studying this enzyme. By understanding the function and regulation of GBA3, researchers can gain insights into metabolic disorders and potentially develop targeted therapies for patients affected by these conditions.
Product Name:
GBA3 Rabbit Polyclonal Antibody
SKU:
CAB7827
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-162 of human GBA3 (NP_001121904.1).
The protein encoded by this gene is a cytosolic enzyme that can hydrolyze several types of glycosides. The enzyme has its highest activity at neutral pH and is predominantly expressed in human liver, kidney, intestine, and spleen. This gene is a polymorphic pseudogene, with the most common allele being the functional allele that encodes the full-length protein. Some individuals contain a single nucleotide polymorphism that results in a premature stop codon in the coding region, and therefore this allele is pseudogenic due to the failure to produce a functional full-length protein. Alternative splicing of this gene results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
57733
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GBA3 antibody (CAB7827) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
