The GBA Polyclonal Antibody (CAB16860) is a powerful tool for researchers studying glucocerebrosidase (GBA), an enzyme involved in the breakdown of certain lipids. This antibody, derived from rabbit polyclonal sources, is highly specific to GBA and is ideal for use in various immunological assays, including Western blotting.GBA plays a crucial role in the lysosomal degradation of glucosylceramide, a lipid molecule found in cell membranes. Mutations in the GBA gene are associated with Gaucher disease, a rare lysosomal storage disorder. By targeting GBA with this antibody, researchers can study its expression levels and distribution in different cell types, shedding light on its role in normal cellular processes and disease pathology.
The GBA Polyclonal Antibody is a valuable tool for investigating the function of GBA in various biological contexts, including lysosomal storage disorders, lipid metabolism, and neurodegenerative diseases. Its high specificity and sensitivity make it an essential component of any research project focused on understanding the complex biology of GBA.
Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human Glucosylceramidase beta (Glucosylceramidase beta (GBA)) (NP_000148.2).
This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2629
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Glucosylceramidase beta (Glucosylceramidase beta (GBA)) Rabbit pAb (CAB16860) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
