The GATA5 Polyclonal Antibody (PAC047178) is a crucial tool for researchers studying the GATA5 protein, a transcription factor that plays a key role in heart development and gastrointestinal tract development. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot applications. By targeting the GATA5 protein, this antibody allows for precise detection and analysis in various cell types, making it an essential resource for investigations in cardiovascular and gastrointestinal research.
GATA5 is essential for proper heart and gastrointestinal tract formation, and abnormalities in its expression have been linked to congenital heart defects and gastrointestinal diseases. The pivotal role of GATA5 in these physiological processes highlights its significance as a therapeutic target for addressing cardiac and digestive disorders. Investigating the function of GATA5 is crucial for advancing our understanding of these diseases and developing potential treatment strategies.
Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using PACO47178 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO47178 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Transcription factor required during cardiovascular development. Plays an important role in the transcriptional program(s) that underlies smooth muscle cell diversity (By similarity). Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer.
GATA5: Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity. Rare variants in GATA5 may be a cause of susceptibility to atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Molecular Function:chromatin binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity; zinc ion binding
Biological Process: anatomical structure formation; blood coagulation; cell development; cell fate commitment; heart development; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq, Jul 2008]
