The GATA5 Polyclonal Antibody (PAC017937) is a valuable tool for researchers studying the GATA5 protein, a transcription factor involved in cardiac development and disease. This antibody, generated in-house using rabbits, exhibits high specificity and sensitivity towards GATA5 in human samples, making it suitable for various applications, including Western blotting and immunofluorescence.GATA5 is known for its crucial role in heart development and function, as well as its implication in cardiac disorders such as congenital heart defects and cardiomyopathy.
By targeting GATA5, researchers can gain insight into the molecular mechanisms underlying these conditions and potentially uncover therapeutic targets for cardiovascular diseases.Overall, the GATA5 Polyclonal Antibody is an essential tool for researchers interested in unraveling the functions of GATA5 in cardiac biology and exploring its potential as a therapeutic target in cardiovascular pathology.
Antibody Name:
GATA5 Antibody (PACO17937)
Antibody SKU:
PACO17937
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human GATA5
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO17937(GATA5 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17937(GATA5 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1α (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity.
Synonyms:
GATA binding protein 5
UniProt Protein Function:
GATA5: Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity. Rare variants in GATA5 may be a cause of susceptibility to atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Molecular Function:chromatin binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity; zinc ion binding
Biological Process: anatomical structure formation; blood coagulation; cell development; cell fate commitment; heart development; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq, Jul 2008]
