The GATA5 Polyclonal Antibody (PAC017936) is essential for research involving GATA5, a transcription factor that plays a vital role in various biological processes. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically targets the GATA5 protein, enabling accurate detection and analysis in a variety of cell types, making it an excellent choice for studies in developmental biology and cardiovascular research.GATA5, a member of the GATA family of transcription factors, is known for its role in heart development and cardiac gene regulation. Mutations in GATA5 have been linked to congenital heart defects and other cardiovascular abnormalities, making it a key target for studying heart development and disease.
Research on GATA5 is crucial for gaining insights into cardiac development and potentially developing therapeutic approaches for heart-related conditions.Overall, the GATA5 Polyclonal Antibody is a valuable tool for researchers investigating the functions of GATA5 in various biological processes, particularly in the field of cardiovascular research. Its high reactivity and specificity ensure accurate and reliable results for studies related to heart development, cardiac gene regulation, and cardiovascular disease.
Antibody Name:
GATA5 Antibody (PACO17936)
Antibody SKU:
PACO17936
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:50-1:200
Species Reactivity:
Human
Immunogen:
Synthetic peptide of human GATA5
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17936(GATA5 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using PACO17936(GATA5 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200).
Background:
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1α (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity.
Synonyms:
GATA binding protein 5
UniProt Protein Function:
GATA5: Binds to the functionally important CEF-1 nuclear protein binding site in the cardiac-specific slow/cardiac troponin C transcriptional enhancer. May play an important role in the transcriptional program(s) that underlies smooth muscle cell diversity. Rare variants in GATA5 may be a cause of susceptibility to atrial fibrillation, a common sustained cardiac rhythm disturbance. Atrial fibrillation is characterized by disorganized atrial electrical activity and ineffective atrial contraction promoting blood stasis in the atria and reduces ventricular filling. It can result in palpitations, syncope, thromboembolic stroke, and congestive heart failure.
Molecular Function:chromatin binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity; zinc ion binding
Biological Process: anatomical structure formation; blood coagulation; cell development; cell fate commitment; heart development; organ morphogenesis; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
NCBI Summary:
The protein encoded by this gene is a transcription factor that contains two GATA-type zinc fingers. The encoded protein is known to bind to hepatocyte nuclear factor-1alpha (HNF-1alpha), and this interaction is essential for cooperative activation of the intestinal lactase-phlorizin hydrolase promoter. In other organisms, similar proteins may be involved in the establishment of cardiac smooth muscle cell diversity. [provided by RefSeq, Jul 2008]
