The GART Polyclonal Antibody (CAB3876) is a valuable tool for researchers studying the enzyme GART, which plays a crucial role in purine biosynthesis. This antibody, produced in rabbits, is highly specific to human GART and has been validated for use in Western blot applications. It binds to the GART protein, allowing for accurate detection and analysis in various cell types, making it ideal for studies in biochemistry and cancer research.GART, also known as phosphoribosylglycinamide formyltransferase, is essential for the de novo synthesis of purines, which are vital for DNA and RNA production in cells.
Dysregulation of GART has been implicated in various diseases, including cancer, making it a promising target for therapeutic interventions. Understanding the function of GART is crucial for advancing research in oncology and other related fields, paving the way for the development of novel treatments and diagnostic tools.
Product Name:
GART Rabbit Polyclonal Antibody
SKU:
CAB3876
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human GART (NP_000810.1).
The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
2618
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GART antibody (CAB3876) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
