The GARS Polyclonal Antibody (CAB5435) is a high-quality research tool designed for the study of GARS, a crucial enzyme involved in protein synthesis and aminoacyl-tRNA charging. This antibody, produced in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By targeting the GARS protein, researchers can accurately detect and analyze its expression in various cell types, making it a valuable tool for investigations in molecular biology and neurology.
GARS, also known as glycyl-tRNA synthetase, plays a critical role in ensuring accurate protein translation and maintaining cellular homeostasis. Dysregulation of GARS has been implicated in neurodegenerative disorders such as Charcot-Marie-Tooth disease, highlighting its importance in neurological function. By studying the function and expression of GARS, researchers can gain insights into the underlying mechanisms of these diseases and potentially identify new therapeutic targets for treatment.
Product Name:
GARS Rabbit Polyclonal Antibody
SKU:
CAB5435
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2).
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2617
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GARS Rabbit pAb (CAB5435) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
