The GARS Polyclonal Antibody (CAB13305) is a powerful tool for researchers interested in studying the role of GARS, a key enzyme involved in aminoacylation of tRNAs, in various cellular processes. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples and has been validated for use in Western blot applications. By targeting the GARS protein, this antibody allows for precise detection and analysis in different cell types, making it ideal for investigations in molecular biology and neurodegenerative diseases research.
GARS, also known as glycyl-tRNA synthetase, plays a crucial role in protein synthesis by catalyzing the attachment of glycine to its cognate tRNA molecule. Dysregulation of GARS has been linked to neurological disorders, such as Charcot-Marie-Tooth disease and distal spinal muscular atrophy, highlighting its importance in maintaining proper neuronal function. By elucidating the biological functions of GARS, researchers can gain valuable insights into the pathogenesis of these disorders and potentially identify new therapeutic targets for intervention.
Product Name:
GARS Rabbit Polyclonal Antibody
SKU:
CAB13305
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-370 of human GARS (NP_002038.2).
This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2617
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GARS antibody (CAB13305) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
