The GAR1 Polyclonal Antibody (CAB12748) is a high-quality antibody designed for research involving GAR1, a protein involved in RNA processing and modification. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications such as Western blotting and immunohistochemistry.GAR1 is a critical component of the ribonucleoprotein complex involved in ribosome biogenesis and RNA stability. It plays a crucial role in cell growth and proliferation, making it a valuable target for studies in molecular biology and cancer research.
By detecting and analyzing GAR1 protein levels, researchers can gain insights into its function and potential implications in various cellular processes.Understanding the role of GAR1 in RNA biology and cellular regulation is essential for advancing our knowledge of gene expression and potential therapeutic strategies. The GAR1 Polyclonal Antibody (CAB12748) offers a reliable tool for researchers to explore the functions of GAR1 in different biological contexts and diseases.
Product Name:
GAR1 Rabbit Polyclonal Antibody
SKU:
CAB12748
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-170 of human GAR1 (NP_061856.1).
This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA2 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The encoded protein of this gene contains two glycine- and arginine-rich domains and is related to Saccharomyces cerevisiae Gar1p. Two splice variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
54433
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GAR1 Rabbit pAb (CAB12748) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
