The GAPDH Rabbit Polyclonal Antibody (CABC027) is a valuable tool for researchers studying glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key enzyme involved in glycolysis and cellular energy production. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.GAPDH is a ubiquitous enzyme found in all cells, making it a commonly used loading control in Western blot experiments. Its stable expression levels under normal physiological conditions make it a reliable marker for protein normalization in studies involving gene expression, protein quantification, and molecular biology research.This polyclonal antibody specifically binds to GAPDH, allowing for accurate detection and quantification of the enzyme in various cell types and tissues.
Its application in research areas such as metabolism, cell biology, and cancer makes it an essential tool for investigating the role of GAPDH in disease development and progression.Overall, the GAPDH Rabbit Polyclonal Antibody (CABC027) is a versatile reagent that provides researchers with reliable and reproducible results in their studies of cellular metabolism and energy regulation. Its high sensitivity and specificity make it an invaluable asset for biochemical research and drug discovery efforts aimed at targeting GAPDH-related pathways.
Product Name:
GAPDH Rabbit Polyclonal Antibody
SKU:
CABC027
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GAPDH Rabbit pAb (AC027) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
