The GAPDH Polyclonal Antibody (CABC001) is a high-quality research tool designed for studying glyceraldehyde-3-phosphate dehydrogenase (GAPDH), an enzyme involved in glycolysis and various cellular processes. This antibody, produced in rabbits, exhibits strong reactivity with human samples and is validated for Western blot applications.GAPDH is a multifunctional protein with roles in energy production, metabolism, and cellular signaling. Its dysregulation has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular conditions.
By targeting GAPDH, researchers can explore its functions in different cell types and pathways, providing insights into disease mechanisms and potential therapeutic strategies.Overall, the GAPDH Polyclonal Antibody is a valuable tool for scientists studying metabolism, cellular biology, and disease pathways. Its specificity and sensitivity make it suitable for a wide range of research applications, contributing to advancements in biomedical science and drug discovery.
Product Name:
GAPDH Rabbit Polyclonal Antibody
SKU:
CABC001
Size:
100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2597
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GAPDH Rabbit pAb (AC001) at 1:30000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
