The Gamma-Enolase Polyclonal Antibody (CAB12341) is an essential tool for research involving gamma-enolase, a key enzyme involved in glycolysis and neuronal development. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.Gamma-enolase is a multifunctional protein that plays a crucial role in various biological processes, including metabolism and neurodevelopment. Its dysregulation has been linked to a range of diseases, such as cancer, neurodegenerative disorders, and autoimmune conditions.
By targeting gamma-enolase, researchers can uncover its functional role in different cell types and explore its potential as a therapeutic target.This polyclonal antibody specifically binds to gamma-enolase, enabling precise detection and analysis in various experimental settings. Its versatility and reliability make it an invaluable tool for studies in neurobiology, metabolism, and cancer research. By understanding the mechanisms underlying gamma-enolase activity, researchers can gain insights into disease pathology and identify novel treatment strategies.
Product Name:
ENO2 Rabbit Polyclonal Antibody
SKU:
CAB12341
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2 (NP_001966.1).
WB,1:100 - 1:500 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
NSE; HEL-S-279; ENO2
Positive Sample:
SH-SY5Y,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm.
Calculated MW:
47kDa
Observed MW:
47kDa
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Purification Method:
Affinity purification
Gene ID:
2026
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using ENO2 Rabbit pAb (CAB12341) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
