Anti-GALT Antibody (CAB19903)[KO Validated]
- SKU:
- CAB19903
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
Anti-GALT Antibody (CAB19903)[KO Validated]
The GALT Polyclonal Antibody (CAB19903) is a powerful tool for researchers studying the role of galactose-1-phosphate uridylyltransferase (GALT) in cellular metabolism and disease. This antibody, produced in rabbits, is specifically designed to target GALT, an essential enzyme involved in the galactose metabolism pathway.With high reactivity to human samples, this antibody has been validated for use in Western blot applications, allowing for the detection and analysis of GALT protein levels in various cell types. This makes it an ideal choice for researchers in the fields of biochemistry, molecular biology, and genetics.
GALT is known to play a crucial role in the breakdown of galactose, a simple sugar found in dairy products and other foods. Mutations in the GALT gene can lead to Galactosemia, a rare genetic disorder that affects the body's ability to metabolize galactose. By studying the function of GALT using this antibody, researchers can gain insight into the underlying mechanisms of Galactosemia and potentially develop new therapeutic strategies for treating this condition.
| Antibody Name: | Anti-GALT Antibody [KO Validated] |
| Antibody SKU: | CAB19903 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant protein of human GALT. |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa |
| Immunogen: | Recombinant protein of human GALT. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 2592 |
| Uniprot: | P07902 |
| Cellular Location: | |
| Calculated MW: | 31kDa/43kDa |
| Observed MW: | 43KDa |
![Anti-GALT Antibody [KO Validated] (CAB19903)](https://cdn11.bigcommerce.com/s-h68l9z2lnx/product_images/f/390/A19903_1__61640.jpg) | Western blot analysis of extracts from normal (control) and GALT knockout (KO) HeLa cells, using GALT antibody (CAB19903) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | GALT |
| Background: | Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family. |
| UniProt Protein Details: | Protein type:EC 2.7.7.12; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Motility/polarity/chemotaxis; Transferase Chromosomal Location of Human Ortholog: 9p13 Cellular Component: cytosol; Golgi apparatus Molecular Function:protein binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity Biological Process: galactose catabolic process; galactose metabolic process Disease: Galactosemia |
| NCBI Summary: | Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] |
| UniProt Code: | P07902 |
| NCBI GenInfo Identifier: | 3183522 |
| NCBI Gene ID: | 2592 |
| NCBI Accession: | P07902.3 |
| UniProt Secondary Accession: | P07902,Q14355, Q14356, Q14357, Q14358, Q14359, Q14360 Q14361, Q14363, Q14364, B4E097, E7ET32, |
| UniProt Related Accession: | P07902 |
| Molecular Weight: | 31,107 Da |
| NCBI Full Name: | Galactose-1-phosphate uridylyltransferase |
| NCBI Synonym Full Names: | galactose-1-phosphate uridylyltransferase |
| NCBI Official Symbol: | GALTÂ Â |
| NCBI Protein Information: | galactose-1-phosphate uridylyltransferase |
| UniProt Protein Name: | Galactose-1-phosphate uridylyltransferase |
| UniProt Synonym Protein Names: | UDP-glucose--hexose-1-phosphate uridylyltransferase |
| Protein Family: | Gallate transporter |
| UniProt Gene Name: | GALTÂ Â |
| UniProt Entry Name: | GALT_HUMAN |
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