The GalNS Polyclonal Antibody (CAB5461) is specially developed for researchers studying GalNS, a key enzyme involved in glycosaminoglycan metabolism. This antibody, produced in rabbits, shows high reactivity with human samples and is ideal for use in Western blot applications. By binding specifically to the GalNS protein, researchers are able to detect and analyze its expression in a variety of cell types.GalNS, also known as N-acetylgalactosamine 6-sulfatase, plays a crucial role in the breakdown of glycosaminoglycans within the body. Dysregulation of GalNS function has been linked to various genetic disorders, including mucopolysaccharidosis, highlighting its importance in maintaining normal cellular function.
By investigating the role of GalNS in disease pathology, researchers can gain insights into potential therapeutic targets for rare genetic conditions.The GalNS Polyclonal Antibody is a valuable tool for researchers interested in studying glycosaminoglycan metabolism and its implications in human health. Its use in Western blotting allows for precise detection of GalNS protein levels, facilitating a better understanding of its function in health and disease.
Product Name:
GALNS Rabbit Polyclonal Antibody
SKU:
CAB5461
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 353-522 of human GALNS (NP_000503.1).
This gene encodes N-acetylgalactosamine-6-sulfatase which is a lysosomal exohydrolase required for the degradation of the glycosaminoglycans, keratan sulfate, and chondroitin 6-sulfate. Sequence alterations including point, missense and nonsense mutations, as well as those that affect splicing, result in a deficiency of this enzyme. Deficiencies of this enzyme lead to Morquio A syndrome, a lysosomal storage disorder.
Purification Method:
Affinity purification
Gene ID:
2588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GALNS antibody (CAB5461) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
