Anti-GAK Antibody (PACO54786)

Antibody Name:	GAK Antibody (PACO54786)
Antibody SKU:	PACO54786
Size:	50ug
Host Species:	Rabbit
Tested Applications:	ELISA, IHC, IF
Recommended Dilutions:	ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:	Human
Immunogen:	Recombinant Human Cyclin-G-associated kinase protein (939-1086AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	Immunohistochemistry of paraffin-embedded human testis tissue using PACO54786 at dilution of 1:100.
	Immunofluorescence staining of HepG2 cells with PACO54786 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Background:	Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Synonyms:	Cyclin-G-associated kinase (EC 2.7.11.1), GAK

UniProt Protein Function:	GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NAK familyChromosomal Location of Human Ortholog: 4p16Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; vesicleMolecular Function: protein bindingBiological Process: Golgi organization and biogenesis
UniProt Protein Details:
NCBI Summary:	In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:	O14976
NCBI GenInfo Identifier:	17375734
NCBI Gene ID:	2580
NCBI Accession:	O14976.2
UniProt Secondary Accession:	O14976,Q5U4P5, Q9BVY6
UniProt Related Accession:	O14976
Molecular Weight:	134,464 Da
NCBI Full Name:	Cyclin-G-associated kinase
NCBI Synonym Full Names:	cyclin G associated kinase
NCBI Official Symbol:	GAK
NCBI Official Synonym Symbols:	DNAJ26; DNAJC26
NCBI Protein Information:	cyclin-G-associated kinase
UniProt Protein Name:	Cyclin-G-associated kinase
UniProt Synonym Protein Names:
Protein Family:	Cyclin-G-associated kinase
UniProt Gene Name:	GAK
UniProt Entry Name:	GAK_HUMAN

Antibodies

GAK Antibody (PACO00833)

GAK Antibody (PACO09395)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)