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GAK Antibody (PACO54786)

SKU:
PACO54786
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
IHC
IF
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • GAK Antibody PACO54786
  • GAK Antibody PACO54786
€339

Description

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GAK Antibody (PACO54786)

The GAK Polyclonal Antibody (PACO54786) is a valuable tool for researchers studying the protein GAK, which plays a key role in various cellular processes including clathrin-mediated endocytosis and mitotic spindle assembly. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.GAK is a crucial regulator of cell division and vesicle trafficking, making it a target of interest in cancer research and drug development. By binding specifically to GAK, this antibody enables researchers to detect and analyze the protein in a variety of cell types, providing valuable insights into its function and potential therapeutic applications.

With its versatility and reliability, the GAK Polyclonal Antibody is an essential tool for scientists studying molecular mechanisms underlying cell division, endocytosis, and other cellular processes influenced by GAK activity. Its use in immunology and cancer research makes it a valuable asset for advancing our understanding of disease pathology and developing targeted therapies.

Antibody Name:GAK Antibody (PACO54786)
Antibody SKU:PACO54786
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA, IHC, IF
Recommended Dilutions:ELISA:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Recombinant Human Cyclin-G-associated kinase protein (939-1086AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageImmunohistochemistry of paraffin-embedded human testis tissue using PACO54786 at dilution of 1:100.
Product ImageImmunofluorescence staining of HepG2 cells with PACO54786 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.
Synonyms:Cyclin-G-associated kinase (EC 2.7.11.1), GAK
UniProt Protein Function:GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NAK familyChromosomal Location of Human Ortholog: 4p16Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; vesicleMolecular Function: protein bindingBiological Process: Golgi organization and biogenesis
UniProt Protein Details:
NCBI Summary:In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
UniProt Code:O14976
NCBI GenInfo Identifier:17375734
NCBI Gene ID:2580
NCBI Accession:O14976.2
UniProt Secondary Accession:O14976,Q5U4P5, Q9BVY6
UniProt Related Accession:O14976
Molecular Weight:134,464 Da
NCBI Full Name:Cyclin-G-associated kinase
NCBI Synonym Full Names:cyclin G associated kinase
NCBI Official Symbol:GAK  
NCBI Official Synonym Symbols:DNAJ26; DNAJC26  
NCBI Protein Information:cyclin-G-associated kinase
UniProt Protein Name:Cyclin-G-associated kinase
UniProt Synonym Protein Names:
Protein Family:Cyclin-G-associated kinase
UniProt Gene Name:GAK  
UniProt Entry Name:GAK_HUMAN
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Secondary Antibody
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