The GAK Polyclonal Antibody (PACO00833) is a valuable tool for researchers studying the protein GAK, which plays a key role in regulating the process of clathrin-mediated endocytosis. This antibody, generated in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.GAK, also known as cyclin G-associated kinase, is essential for the regulation of vesicle trafficking and receptor internalization, making it a crucial player in cellular processes such as signal transduction and protein sorting. Its involvement in endocytosis also suggests potential implications in diseases related to dysfunctional cellular trafficking, such as neurodegenerative disorders and certain types of cancer.
By utilizing the GAK Polyclonal Antibody, researchers can study the expression and localization of GAK in various cell types, providing insights into its role in normal cellular function and disease pathology. This antibody is a valuable tool for investigations in cell biology, molecular biology, and cancer research, facilitating a better understanding of the complex mechanisms underlying endocytosis and its implications in human health and disease.
Antibody Name:
GAK Antibody
Antibody SKU:
PACO00833
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC, IF
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:
Human, Mouse
Immunogen:
synthesized peptide derived from the N-terminal region of human GAK.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
GAK; Cyclin-G-associated kinase
UniProt Protein Function:
GAK: Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; NAK familyChromosomal Location of Human Ortholog: 4p16Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; membrane; vesicleMolecular Function: protein bindingBiological Process: Golgi organization and biogenesis
UniProt Protein Details:
NCBI Summary:
In all eukaryotes, the cell cycle is governed by cyclin-dependent protein kinases (CDKs), whose activities are regulated by cyclins and CDK inhibitors in a diverse array of mechanisms that involve the control of phosphorylation and dephosphorylation of Ser, Thr or Tyr residues. Cyclins are molecules that possess a consensus domain called the 'cyclin box.' In mammalian cells, 9 cyclin species have been identified, and they are referred to as cyclins A through I. Cyclin G is a direct transcriptional target of the p53 tumor suppressor gene product and thus functions downstream of p53. GAK is an association partner of cyclin G and CDK5. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]
