The GADD45A Polyclonal Antibody (CAB1797) is specifically designed for research involving the growth arrest and DNA damage-inducible protein 45A (GADD45A), a key player in cellular stress response pathways. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in Western blot applications.GADD45A is known to be involved in cell cycle regulation, DNA repair, and apoptosis, making it a critical protein in maintaining genomic stability and promoting cellular survival under stress conditions.
The antibody binds specifically to GADD45A protein, allowing for precise detection and analysis in various cell types.Research on GADD45A is essential for understanding its role in processes like DNA damage repair, cell cycle arrest, and cell survival, making it a valuable target for studies in cancer research, genomics, and molecular biology. The GADD45A Polyclonal Antibody is a powerful tool for investigating the function and regulation of this important protein in various biological contexts.
Product Name:
GADD45A Rabbit Polyclonal Antibody
SKU:
CAB1797
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-165 of human GADD45A (NP_001915.1).
This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
1647
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GADD45A Rabbit pAb (CAB1797) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
