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Anti-GADD45A Antibody (CAB13486)

SKU:
CAB13486
Product Type:
Antibody
Reactivity:
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Cycle
$167 - $503
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-GADD45A Antibody
Antibody SKU:CAB13486
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse, Rat
Positive Samples:Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human GADD45A (NP_001915.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MTLE EFSA GEQK TERM DKVG DALE EVLS KALS QRTI TVGV YEAA KLLN VDPD NVVL CLLA ADED DDRD VALQ IHFT LIQA FCCE NDIN ILRV SNPG RLAE LLLL ETDA GPAA SEGA EQPP DLHC VLVT NPHS SQWK DPAL SQLI CFCR ESRY MDQW VPVI NLPE R
Gene ID:1647
Uniprot:P24522
Cellular Location:Nucleus
Calculated MW:14kDa/18kDa
Observed MW:18kDa
Synonyms:DDIT1, GADD45, GADD45A
Background:This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
UniProt Protein Function:GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; DNA repair, damage; Apoptosis; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function:protein binding

Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity

NCBI Summary:This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]
UniProt Code:P24522
NCBI GenInfo Identifier:120751
NCBI Gene ID:1647
NCBI Accession:P24522.1
UniProt Secondary Accession:P24522,Q5TCA7, Q5TCA8,
UniProt Related Accession:P24522
Molecular Weight:14,622 Da
NCBI Full Name:Growth arrest and DNA damage-inducible protein GADD45 alpha
NCBI Synonym Full Names:growth arrest and DNA damage inducible alpha
NCBI Official Symbol:GADD45A  
NCBI Official Synonym Symbols:DDIT1; GADD45  
NCBI Protein Information:growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Protein Name:Growth arrest and DNA damage-inducible protein GADD45 alpha
UniProt Synonym Protein Names:DNA damage-inducible transcript 1 protein; DDIT-1
Protein Family:Growth arrest and DNA damage-inducible protein
UniProt Gene Name:GADD45A  
UniProt Entry Name:GA45A_HUMAN
Anti-GADD45A Antibody (CAB13486)Western blot analysis of extracts of mouse liver, using GADD45A antibody (CAB13486) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

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