The GAD2 Polyclonal Antibody (CAB0971) is a valuable tool for researchers investigating GAD2, an enzyme involved in the synthesis of the neurotransmitter gamma-aminobutyric acid (GABA). This antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications.GAD2 plays a crucial role in the regulation of GABA levels in the brain, impacting processes such as neurotransmission, synaptic plasticity, and neuronal excitability. Dysregulation of GABA signaling has been implicated in various neurological disorders, including epilepsy, anxiety, and schizophrenia, making GAD2 an important target for further study.
The GAD2 Polyclonal Antibody binds specifically to the GAD2 protein, allowing for precise detection and analysis in a variety of cell types. Its applicability in immunology and neuroscience research makes it an essential tool for understanding the role of GAD2 in both normal brain function and disease states. Researchers interested in exploring the mechanisms of GABAergic signaling and developing novel therapeutic strategies will find this antibody invaluable in their work.
Product Name:
GAD2 Rabbit Polyclonal Antibody
SKU:
CAB0971
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 401-585 of human GAD65/GAD2 (NP_001127838.1).
This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Purification Method:
Affinity purification
Gene ID:
2572
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GAD65/GAD2 antibody (CAB0971) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
