GAD1/GAD2 Antibody (PACO06500)
- SKU:
- PACO06500
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IHC
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
GAD1/GAD2 Antibody (PACO06500)
The GAD1/GAD2 Antibody (PACO06500) is a high-quality polyclonal antibody designed for use in research involving the GAD1 and GAD2 proteins, which are key enzymes in the synthesis of gamma-aminobutyric acid (GABA) in the brain. This antibody is raised in rabbits and has been validated for use in Western blot applications, allowing for accurate detection and analysis of GAD1 and GAD2 levels in various samples.GAD1 and GAD2 are critical for the regulation of GABA levels in the brain, which plays a crucial role in neurotransmission and neuronal excitability. Dysregulation of GABA synthesis has been implicated in a variety of neurological disorders, including epilepsy, anxiety, and depression.
Studying the expression and activity of GAD1 and GAD2 using this antibody can provide valuable insights into the mechanisms underlying these conditions and may lead to the development of novel therapeutic strategies.Furthermore, the GAD1/GAD2 Antibody (PACO06500) is highly specific for human samples, making it an ideal tool for research in the fields of neuroscience and neurology. By accurately detecting and quantifying GAD1 and GAD2 levels, researchers can further our understanding of GABAergic neurotransmission and potentially identify new targets for the treatment of neurological disorders.
| Antibody Name: | GAD1/GAD2 Antibody (PACO06500) |
| Antibody SKU: | PACO06500 |
| Size: | 50ug |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IHC, IF |
| Recommended Dilutions: | ELISA:1:5000, WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Synthesized peptide derived from the C-terminal region of human GAD-65/67. |
| Form: | Liquid |
| Storage Buffer: | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot analysis of various cells using GAD-65/67 Polyclonal Antibody. |
 | Western Blot analysis of mouse cells using GAD-65/67 Polyclonal Antibody. |
| Synonyms: | GAD1; GAD; GAD67; Glutamate decarboxylase 1; 67 kDa glutamic acid, decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform; GAD2; GAD65; Glutamate decarboxylase 2; 65 kDa glutamic acid, decarboxylase; GAD-65; Glutamate decarboxylase 65 |
| UniProt Protein Function: | GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Mitochondrial; Lyase Chromosomal Location of Human Ortholog: 2q31 Cellular Component: plasma membrane; intracellular; vesicle membrane Molecular Function:protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding Biological Process: response to drug; glutamate catabolic process; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; gamma-aminobutyric acid biosynthetic process; protein-pyridoxal-5-phosphate linkage; neurotransmitter biosynthetic process Disease: Cerebral Palsy, Spastic Quadriplegic, 1 |
| NCBI Summary: | This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99259 |
| NCBI GenInfo Identifier: | 1352213 |
| NCBI Gene ID: | 2571 |
| NCBI Accession: | Q99259.1 |
| UniProt Secondary Accession: | Q99259,Q49AK1, Q53TQ7, Q9BU91, Q9UHH4, |
| UniProt Related Accession: | Q99259 |
| Molecular Weight: | 66,897 Da |
| NCBI Full Name: | Glutamate decarboxylase 1 |
| NCBI Synonym Full Names: | glutamate decarboxylase 1 (brain, 67kDa) |
| NCBI Official Symbol: | GAD1Â Â |
| NCBI Official Synonym Symbols: | GAD; SCP; CPSQ1Â Â |
| NCBI Protein Information: | glutamate decarboxylase 1; GAD-67; 67 kDa glutamic acid decarboxylase; glutamate decarboxylase 67 kDa isoform |
| UniProt Protein Name: | Glutamate decarboxylase 1 |
| UniProt Synonym Protein Names: | 67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform |
| Protein Family: | Glutamate decarboxylase |
| UniProt Gene Name: | GAD1Â Â |
| UniProt Entry Name: | DCE1_HUMAN |
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