The GABARAP Monoclonal Antibody (CAB4335) is a high-quality antibody developed for reliable detection and analysis of target proteins. Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. RRID AB_2863236 Gene ID 11337 Swiss Prot Synonym MM46; ATG8A; GABARAP-a; GABARAP
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
GABARAP Monoclonal Antibody
SKU:
CAB4335
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC1057
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MM46, ATG8A, GABARAP-a, GABARAP
Positive Sample:
293T treated with Chloroquine, NIH/3T3 treated with Chloroquine, C6 treated with Chloroquine
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. RRID AB_2863236 Gene ID 11337 Swiss Prot Synonym MM46; ATG8A; GABARAP-a; GABARAP
Purification Method:
Affinity purification
Gene ID:
11337
RRID:
AB_2863236
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GABARAP Rabbit mAb (CAB4335) at 1:1000 dilution. 293T、NIH/3T3 and C6 cells were treated with Chloroquine (50 μM) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
