G6PDH Inhibitor Screening Kit (BA0194)

About Glucose-6-Phosphate Dehydrogenase:

GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics.

How our G6PDH Assay Kits Work:

Assay Genie's non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.

Key Features:

• Safe. Non-radioactive assay.
• Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed.

Materials required, but not provided:

Purified G6PDH (e.g. Calzyme Cat# 078A0020) and if desired a control Zn2+ inhibitor (e.g. Zn(NO3)2 · 6H2O, Sigma Cat# 228737). Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat bottom 96-well plates (e.g. VWR cat# 82050-760), and plate reader.