The G6PD Polyclonal Antibody (CAB1537) is a valuable tool for researchers studying glucose-6-phosphate dehydrogenase (G6PD), an enzyme involved in the pentose phosphate pathway and important for maintaining cellular redox balance. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the G6PD protein, this antibody allows for the detection and analysis of G6PD expression in various cell types.G6PD plays a crucial role in protecting cells from oxidative damage and is associated with conditions such as favism and G6PD deficiency.
Studies on G6PD are of particular interest in the fields of biochemistry, metabolism, and genetics. The G6PD Polyclonal Antibody is an essential tool for investigating the function and regulation of G6PD in normal physiological processes and disease states, making it a valuable asset for research in areas such as oxidative stress, metabolic disorders, and drug development.
Product Name:
G6PD Rabbit Polyclonal Antibody
SKU:
CAB1537
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 195-515 of human G6PD (NP_001035810.1).
cytoplasm, cytoplasmic side of plasma membrane, cytosol, extracellular exosome, nucleus
Calculated MW:
59kDa
Observed MW:
59kDa
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2539
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of MCF7, using G6PD antibody (CAB1537) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
