The G6PD Polyclonal Antibody (CAB13983) is a valuable tool for researchers studying glucose-6-phosphate dehydrogenase (G6PD), an enzyme involved in the Pentose Phosphate Pathway (PPP) and redox regulation. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.G6PD is a crucial enzyme that plays a key role in the production of NADPH, an important molecule for cellular antioxidant defenses and biosynthetic pathways. Dysregulation of G6PD activity has been linked to various diseases, including glucose-6-phosphate dehydrogenase deficiency (G6PD deficiency), which can lead to hemolytic anemia in patients.
By utilizing the G6PD Polyclonal Antibody, researchers can accurately detect and quantify G6PD protein levels in different cell types and tissue samples. This antibody is essential for further understanding the role of G6PD in redox homeostasis, cellular metabolism, and disease pathogenesis. Its application extends to studies in cancer research, metabolism, and oxidative stress, offering valuable insights into G6PD function and its potential as a therapeutic target in various pathological conditions.
Product Name:
G6PD Rabbit Polyclonal Antibody
SKU:
CAB13983
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 415-515 of human G6PD (NP_001035810.1).
cytoplasm, cytoplasmic side of plasma membrane, cytosol, extracellular exosome, nucleus
Calculated MW:
59kDa
Observed MW:
60kDa
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2539
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and G6PD knockdown (KD) HeLa cells using G6PD Rabbit pAb (CAB13983) at 1:800 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime: 10s.
