The G6PC Polyclonal Antibody (PAB10001) is a valuable tool for researchers studying the enzyme glucose-6-phosphatase (G6PC), which plays a crucial role in glucose metabolism and glycogenolysis. This antibody, generated in rabbits, is highly specific to human samples and is suitable for use in Western blot applications. By binding to the G6PC protein, this antibody allows for the detection and analysis of G6PC in various cell types, making it an ideal choice for research in metabolism, diabetes, and liver disorders.
G6PC is an essential enzyme that catalyzes the final step in gluconeogenesis and glycogenolysis, making it a key regulator of blood glucose levels. Dysregulation of G6PC activity is associated with diabetes and other metabolic disorders, making it an attractive target for therapeutic interventions. By studying the expression and function of G6PC using this antibody, researchers can gain valuable insights into the mechanisms underlying these diseases and potentially identify new therapeutic strategies.
Product Name:
G6PC Polyclonal Antibody
SKU:
CAB21168
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human G6PC (NP_000142.2).
Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.
Purification Method:
Affinity purification
Gene ID:
2538
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Huh-7 cells using G6PC Rabbit pAb(CAB21168) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
