The Frizzled-6 Polyclonal Antibody (CAB10503) is a valuable tool for researchers studying the Frizzled-6 protein, a member of the Frizzled family known for its role in the Wnt signaling pathway. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications.Frizzled-6 is a transmembrane receptor that plays a crucial role in cell signaling and development, particularly in processes such as cell proliferation, migration, and differentiation. Dysregulation of the Wnt signaling pathway, in which Frizzled-6 is involved, has been linked to various diseases including cancer, making this protein an important target for research.
By using the Frizzled-6 Polyclonal Antibody, researchers can effectively detect and analyze the expression of Frizzled-6 in various cell types, aiding in the understanding of its function and potential involvement in disease processes. This antibody is a valuable tool for studies in developmental biology, cancer research, and other fields where Wnt signaling is of interest.
Product Name:
FZD6 Rabbit Polyclonal Antibody
SKU:
CAB10503
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-201 of human FZD6 (NP_003497.2).
This gene represents a member of the 'frizzled' gene family, which encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The protein encoded by this family member contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, and seven transmembrane domains, but unlike other family members, this protein does not contain a C-terminal PDZ domain-binding motif. This protein functions as a negative regulator of the canonical Wnt/beta-catenin signaling cascade, thereby inhibiting the processes that trigger oncogenic transformation, cell proliferation, and inhibition of apoptosis. Alternative splicing results in multiple transcript variants, some of which do not encode a protein with a predicted signal peptide.
Purification Method:
Affinity purification
Gene ID:
8323
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell line, using FZD6 antibody (CAB10503) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
