The FYCO1 Polyclonal Antibody (PACO22338) is a valuable tool for researchers studying FYCO1, a protein involved in autophagy and intracellular trafficking processes. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting FYCO1 in human samples, making it ideal for Western blot applications.FYCO1, also known as FYVE and coiled-coil domain containing 1, plays a key role in autophagosome biogenesis and maturation by interacting with microtubule-associated proteins and autophagy-related proteins.
Its involvement in intracellular transport processes makes it a target of interest in the study of various diseases, including neurodegenerative disorders and cancer.By using the FYCO1 Polyclonal Antibody, researchers can investigate the functions and regulation of FYCO1 in different cell types and experimental conditions. This antibody provides a reliable tool for studying the role of FYCO1 in autophagy pathways, allowing for deeper insights into cellular processes and potential therapeutic targets.
Antibody Name:
FYCO1 Antibody (PACO22338)
Antibody SKU:
PACO22338
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from Internal of human FYCO1.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from JurKat cells, using FYCO1 antibody.
Background:
May mediate microtubule plus end-directed vesicle transport.
Synonyms:
DKFZp779K1152; FLJ13335; FYCO1; FYVE and coiled-coil domain containing 1; FYVE and coiled-coil domain-containing protein 1
UniProt Protein Function:
FYCO1: May mediate microtubule plus end-directed vesicle transport. Defects in FYCO1 are the cause of cataract congenital autosomal recessive type 2 (CATC2). An opacification of the crystalline lens of the eye becoming evident at birth or in infancy. It frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Pathogenic mutations in FYCO1 can affect intracellular transport of autophagocytic vesicles from the perinuclear area to the periphery, leading to an accumulation of large numbers of vesicles and hence loss of lens transparency (PubMed:21636066). 3 isoforms of the human protein are produced by alternative splicing.Protein type: AutophagyChromosomal Location of Human Ortholog: 3p21.31Cellular Component: autophagic vacuole; cytoplasmic vesicle; integral to membrane; intracellular membrane-bound organelle; late endosome; lysosome; membraneMolecular Function: metal ion binding; protein bindingDisease: Cataract 18
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein that contains a RUN domain, FYVE-type zinc finger domain and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P) and the autophagosome marker LC3. Mutations in this gene are a cause of autosomal recessive congenital cataract-2 (CATC2). [provided by RefSeq, Dec 2011]
