The FXN Polyclonal Antibody (CAB16853) is a research tool designed for studying the protein frataxin (FXN), a key player in the pathogenesis of Friedreich's ataxia, a progressive neurodegenerative disorder. This antibody, derived from rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.Frataxin is a mitochondrial protein involved in iron homeostasis and the assembly of iron-sulfur clusters. Dysregulation of FXN expression leads to mitochondrial dysfunction and oxidative stress, contributing to the development of Friedreich's ataxia. The FXN Polyclonal Antibody enables precise detection and analysis of frataxin levels in various experimental settings, making it a valuable tool for researchers investigating mitochondrial disorders and neurodegenerative diseases.
By targeting FXN, researchers can gain insights into the molecular mechanisms underlying Friedreich's ataxia and potentially identify therapeutic strategies that restore mitochondrial function and alleviate disease symptoms. The FXN Polyclonal Antibody offers a reliable means of exploring the role of frataxin in health and disease, paving the way for advancements in the field of mitochondrial biology and neurological research.
Product Name:
FXN Rabbit Polyclonal Antibody
SKU:
CAB16853
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 42-210 of human FXN (NP_000135.2).
This nuclear gene encodes a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA from 8-33 repeats to >90 repeats results in Friedreich ataxia. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2395
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using FXN Rabbit pAb (CAB16853) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
