The FUT8 Polyclonal Antibody (CAB12933) is a valuable tool for researchers studying FUT8, a key enzyme involved in the glycosylation of proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the FUT8 protein, this antibody enables the detection and analysis of FUT8 in various cell types, making it ideal for investigations in glycobiology and cancer research.FUT8, also known as α1,6-fucosyltransferase, plays a crucial role in the catalysis of core fucosylation, a type of protein glycosylation known to modulate various cellular processes.
Dysregulation of FUT8 has been implicated in cancer progression, making it a promising target for the development of therapeutic interventions. Understanding the function of FUT8 and its impact on glycosylation pathways is essential for advancing our knowledge of disease mechanisms and identifying potential drug targets.
Product Name:
FUT8 Rabbit Polyclonal Antibody
SKU:
CAB12933
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 376-575 of human FUT8 (NP_835367.1).
Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein.
Calculated MW:
67kDa
Observed MW:
75kDa
This gene encodes an enzyme belonging to the family of fucosyltransferases. The product of this gene catalyzes the transfer of fucose from GDP-fucose to N-linked type complex glycopeptides. This enzyme is distinct from other fucosyltransferases which catalyze alpha1-2, alpha1-3, and alpha1-4 fucose addition. The expression of this gene may contribute to the malignancy of cancer cells and to their invasive and metastatic capabilities. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2530
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FUT8 Rabbit pAb (CAB12933) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
