The Furin Rabbit Monoclonal Antibody (CAB5043) is a cutting-edge tool for researchers studying furin, a widely expressed proprotein convertase involved in the processing of precursor proteins. This antibody, developed using rabbit monoclonal technology, exhibits high specificity and sensitivity for detecting furin in various biological samples.Furin plays a crucial role in the activation of numerous proteins and peptides, making it a key player in processes such as viral infection, cellular signaling, and tumorigenesis. The CAB5043 antibody enables precise detection and quantification of furin, allowing researchers to delve deeper into its functions and implications in various biological pathways.
This monoclonal antibody is validated for use in applications such as immunohistochemistry, immunofluorescence, and Western blotting, providing versatile options for studying furin expression and localization in different experimental settings. With its superior performance and reliability, the CAB5043 antibody is an indispensable tool for investigations in molecular biology, cell biology, and biomedical research.
Product Name:
Furin Rabbit Monoclonal Antibody
SKU:
CAB5043
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Furin (P09958).
Cell membrane, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network membrane, Secreted, Endosome membrane
Calculated MW:
87kDa
Observed MW:
90kDa
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5045
Clone Number:
ARC1221
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using Furin Rabbit mAb (CAB5043) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
