The Furin Polyclonal Antibody (CAB7445) is a highly specific and sensitive tool for research involving the furin protein. Furin is a protease enzyme involved in the processing of precursor proteins into their active forms, making it a crucial player in various cellular processes. This antibody, generated in rabbits, is optimized for use in Western blot and immunohistochemistry applications, allowing for precise detection and localization of furin in cell and tissue samples.
Furin is known to be involved in the activation of numerous proteins, including growth factors, receptors, and viral proteins, making it a key target for studying molecular pathways in diseases such as cancer, viral infections, and endocrine disorders. The ability of the Furin Polyclonal Antibody to specifically recognize and bind to furin protein makes it an essential tool for researchers aiming to understand the role and regulation of furin in health and disease.
Product Name:
Furin Rabbit Polyclonal Antibody
SKU:
CAB7445
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Furin (NP_002560.1).
Cell membrane, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
87kDa
Observed MW:
90-110kDa
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. Like other members of this convertase family, the product of this gene specifically cleaves substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140, and may play a role in tumor progression. Unlike SARS-CoV and other coronaviruses, the spike protein of SARS-CoV-2 is thought to be uniquely cleaved by this protease. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
5045
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from 293F cells, using Furin Rabbit pAb (CAB7445) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
