The FUBP1 Polyclonal Antibody (CAB5587) is a valuable tool for researchers studying FUBP1, a protein involved in regulating gene expression and DNA replication. This antibody, produced in rabbits, exhibits high reactivity with human samples and is validated for use in Western blotting applications.FUBP1, also known as far upstream element-binding protein 1, plays a key role in controlling the expression of genes involved in cell growth, differentiation, and survival. Dysregulation of FUBP1 has been implicated in various diseases, including cancer, making it an attractive target for research aimed at understanding the molecular mechanisms underlying these conditions.
By specifically binding to the FUBP1 protein, this antibody enables researchers to detect and analyze FUBP1 levels in different cell types, facilitating studies in molecular biology, cancer research, and developmental biology. Investigating the function of FUBP1 is essential for uncovering its role in disease pathogenesis and identifying potential therapeutic strategies targeting this protein.
Product Name:
FUBP1 Rabbit Polyclonal Antibody
SKU:
CAB5587
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1 (NP_003893.2).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
FBP; FUBP; hDH V; FUBP1
Positive Sample:
U-87MG,HeLa,HepG2,THP-1,Mouse brain
Conjugate:
Unconjugated
Cellular Localization:
Nucleus.
Calculated MW:
68kDa
Observed MW:
79kDa
The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
8880
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using FUBP1 Rabbit pAb (CAB5587) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
