The FTO Polyclonal Antibody (CAB18672) is a vital tool for researchers studying the FTO protein, an enzyme involved in RNA metabolism and energy homeostasis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the FTO protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in molecular biology and metabolic research.FTO, also known as fat mass and obesity-associated protein, plays a crucial role in regulating energy balance and metabolism by demethylating RNA and affecting gene expression.
Dysregulation of FTO has been linked to obesity, diabetes, and cancer, making it a promising target for therapeutic interventions. Understanding the functions of FTO is essential for developing treatments aimed at metabolic disorders and cancer, as well as for gaining insights into the molecular mechanisms underlying these conditions.
Product Name:
FTO Rabbit Polyclonal Antibody
SKU:
CAB18672
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of zebrafish FTO (XP_009301626.1).
Predicted to enable 2-oxoglutarate-dependent dioxygenase activity and ferrous iron binding activity. Acts upstream of or within several processes, including cilium assembly; left/right pattern formation; and positive regulation of canonical Wnt signaling pathway. Predicted to be located in cytoplasm and nuclear speck. Human ortholog(s) of this gene implicated in obesity. Orthologous to human FTO (FTO alpha-ketoglutarate dependent dioxygenase).
Purification Method:
Affinity purification
Gene ID:
553363
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FTO antibody (CAB18672) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
