The FTO Polyclonal Antibody (CAB1438) is a valuable tool for researchers studying FTO, a protein involved in the regulation of mRNA splicing and fat metabolism. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the FTO protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of molecular biology and metabolic research.
FTO, also known as fat mass and obesity associated protein, plays a crucial role in the regulation of energy metabolism and body weight. Its involvement in mRNA splicing suggests a potential role in gene expression regulation, making it a promising target for understanding diseases like obesity and metabolic disorders. Research on FTO can provide insights into the mechanisms underlying these conditions and potentially lead to the development of new therapeutic interventions.
Product Name:
FTO Rabbit Polyclonal Antibody
SKU:
CAB1438
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 327-504 of human FTO (NP_001073901.1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
GDFD; ALKBH9; BMIQ14; FTO
Positive Sample:
HeLa,293T,SH-SY5Y
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, Nucleus speckle.
Calculated MW:
58kDa
Observed MW:
60kDa
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.
Purification Method:
Affinity purification
Gene ID:
79068
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using FTO Rabbit pAb (CAB1438) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
