fT3 (Free Triiodothyronine) ELISA Kit (AEES00002)
- SKU:
- AEES00002
- Product Type:
- ELISA Kit
- ELISA Type:
- Competitive
- Size:
- 96 Assays
- Sensitivity:
- 0.94 pg/mL
- Range:
- 1.56-100 pg/mL
- Sample Type:
- Serum, plasma and other biological fluids
Description
Product Name: | fT3 (Free Triiodothyronine) ELISA Kit |
SKU: | AEES00002 |
Target: | fT3 (Free Triiodothyronine) |
Size: | 96T |
Assay type: | Competitive-ELISA |
Assay time: | 2.0h |
Sensitivity: | 0.94 pg/mL |
Detection range: | 1.56-100 pg/mL |
Kit component: |
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This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT3. During the reaction, fT3 in the sample or standard competes with a fixed amount of fT3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to fT3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of fT3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity: |
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Recovery: |
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Precision: |
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Sample type &Sample volume: | serum, plasma and other biological fluids; 50μL |
Reproducibility: | Both intra-CV and inter-CV are < 10%. |
Application: | This ELISA kit applies to the in vitro quantitative determination of fT3 concentrations in serum, plasma and other biological fluids. |
Specificity: | This kit recognizes fT3 in samples. No significant cross-reactivity or interference between fT3 and analogues was observed. |