The LTD4 (Leukotriene D4) ELISA Kit is designed for the quantitative detection of Leukotriene D4 (LTD4) levels in various biological samples. LTD4 is a potent inflammatory mediator involved in a wide range of cellular activities, including bronchoconstriction, vascular permeability and leukocyte adhesion. It significantly influences the body's immune response by causing smooth muscle contractions and has been implicated in various pathological conditions like asthma, allergic rhinitis, and cardiovascular diseases. Accurate measurement of LTD4 is crucial for understanding its involvement in disease pathogenesis and for the development of targeted therapeutic strategies.
Assay Genie's LTD4 (Leukotriene D4) ELISA Kit offers exceptional sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under stringent quality control standards, this kit provides robust performance and is easy to use, making it an ideal choice for both research and clinical applications. Trust Assay Genie's LTD4 ELISA Kit for accurate and dependable quantification of this key biomarker in your investigations.
Product Name:
fT3 (Free Triiodothyronine) ELISA Kit
SKU:
AEES00002
Target:
fT3 (Free Triiodothyronine)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT3. During the reaction, fT3 in the sample or standard competes with a fixed amount of fT3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to fT3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of fT3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-98
91-104
88-98
Average (%)
93
96
95
1:4
Range (%)
91-106
90-103
95-105
Average (%)
98
97
100
1:8
Range (%)
92-100
90-104
91-102
Average (%)
95
96
95
1:16
Range (%)
99-110
88-95
88-96
Average (%)
102
92
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-95
91
EDTA plasma (n=5)
96-108
104
Cell culture media (n=5)
101-107
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.16
12.43
47.53
4.84
11.65
49.47
Standard deviation
0.23
0.59
2.44
0.24
0.57
2.4
C V (%)
5.53
4.75
5.13
4.96
4.89
4.85
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of fT3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes fT3 in samples. No significant cross-reactivity or interference between fT3 and analogues was observed.
