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FT Polyclonal Antibody (CAB21280)

SKU:
CAB21280
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Plant - Arabidopsis
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
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Description

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Product Name:FT Polyclonal Antibody
SKU:CAB21280
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Arabidopsis thaliana
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 70-150 of arabidopsis thaliana FT (NP_176726.1).
Sequence:VDPD VPSP SNPH LREY LHWL VTDI PATT GTTF GNEI VCYE NPSP TAGI HRVV FILF RQLG RQTV YAPG WRQN FNTR EFAE I
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:F5I14.3; F5I14_3; FLOWERING LOCUS T; REDUCED STEM BRANCHING 8; RSB8; FT
Positive Sample:Seedling,Before inflorescence
Conjugate:Unconjugated
Cellular Localization:cytoplasm, endoplasmic reticulum, nucleus
Calculated MW:20kDa
Observed MW:20kDa

FT, together with LFY, promotes flowering and is antagonistic with its homologous gene, TERMINAL FLOWER1 (TFL1). FT is expressed in leaves and is induced by long day treatment. Either the FT mRNA or protein is translocated to the shoot apex where it induces its own expression. Recent data suggests that FT protein acts as a long-range signal. FT is a target of CO and acts upstream of SOC1.

Purification Method:Affinity purification
Gene ID:842859
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various tissues from Arabidopsis thaliana, using FT Rabbit pAb (CAB21280) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.

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