The Follistatin Polyclonal Antibody (CAB2936) is a vital tool for researchers studying the protein Follistatin, known for its role in regulating cell growth and differentiation. Raised in rabbits, this antibody exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Follistatin protein, this antibody enables the detection and analysis of Follistatin in various cell types, making it ideal for studies in developmental biology, reproductive health, and muscle growth research.
Follistatin is a multifunctional protein that plays a crucial role in various physiological processes, including muscle development, reproductive function, and wound healing. It is particularly important in the regulation of follicle-stimulating hormone (FSH) and activin, making it a valuable target for research into fertility, muscular dystrophy, and other growth-related disorders. Understanding the mechanisms and functions of Follistatin is essential for developing potential therapies and interventions in these areas.
Product Name:
FST Rabbit Polyclonal Antibody
SKU:
CAB2936
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human FST (P19883).
Sequence:
AACS SGVL LEVK HSGS CNSI SEDT EEEE EDED QDYS FPIS SILE W
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
FS; FST
Positive Sample:
HepG2,Rat pancreas
Conjugate:
Unconjugated
Cellular Localization:
Secreted.
Calculated MW:
38kDa
Observed MW:
38kDa
Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin.
Purification Method:
Affinity purification
Gene ID:
10468
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FST antibody (CAB2936) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
