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Fructose Fluorometric Assay Kit (MAES0289)

SKU:
MAES0289
Product Type:
Assay
Instrument:
Fluorescence microplate reader
Detection Method:
Fluorescence method
Sample Type:
Serum, Plasma, Tissue
Sensitivity:
0.72 μmol/L
$587
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Description

system_update_altDatasheet
Product Name: Fructose Fluorometric Assay Kit
Product Code: MAES0289
Sample Type: Serum, Plasma, Tissue
Assay Type: Quantitative
Assay Time: 150 minutes
Detection Instrument: Fluorescence microplate reader (Ex/Em=530 nm/590 nm)
Detection Method: Fluorescence method
Sensitivity: 0.72 μmol/L
Detection Range: 0.72-500 μmol/L
Intra-Assay Precision: Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/L) 50.0 150.0 350.0
%CV 1.7 2.3 3.1
Inter-Assay Precision: Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
Parameters Sample 1 Sample 2 Sample 3
Mean (μmol/L) 50.0 150.0 350.0
%CV 3.2 5.2 7.3
Recovery: Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.
Standard 1 Standard 2 Standard 3
Expected Conc. (μmol/L) 50.0 150.0 350.0
Observed Conc. (μmol/L) 48.5 142.5 388.5
Recovery Rate(%) 97 96 111
Standard Curve: As the fluorescence value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve is provided as below for reference only.
Fructose Fluorometric Assay Kit (MAES0289)

Fructose is the most common hexketoose, which is often present in large amounts as a free monosaccharide in fruits and honey. A large number of epidemiological data and experimental studies have shown that excessive fructose intake may be an important factor in the increased incidence of metabolic diseases. Fructose can produce a specific product under the action of enzymes, which reacts with the chromogenic agent to produce a fluorescent substance (Ex/Em: 530 nm/590 nm).

Storage: -20°C
Valid Period: 12 months
Other Detection Instrument: Incubator (37℃), Vortex mixer
Other Reagents Required: Normal saline (0.9% NaCl)

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