The Frizzled 7 Polyclonal Antibody (CAB4213) is a valuable tool for researchers studying the Frizzled 7 protein, a member of the Frizzled family of G protein-coupled receptors that plays a key role in the Wnt signaling pathway. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to Frizzled 7, this antibody enables the detection and analysis of the protein in various cell types, allowing for detailed investigation into its function and regulation.Frizzled 7 is known to be involved in a wide range of cellular processes, including cell proliferation, differentiation, and polarity, as well as embryonic development and tissue homeostasis.
Dysregulation of Frizzled 7 signaling has been implicated in various diseases, including cancer, neurodevelopmental disorders, and cardiovascular conditions. By studying the role of Frizzled 7 using this antibody, researchers can gain valuable insights into its contribution to these pathological processes and potential therapeutic strategies targeting this pathway.
Product Name:
FZD7 Rabbit Polyclonal Antibody
SKU:
CAB4213
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FZD7 (NP_003498.1).
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.
Purification Method:
Affinity purification
Gene ID:
8324
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FZD7 antibody (CAB4213) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
