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Anti-FZD7 Antibody (CAB4213)

SKU:
CAB4213
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
€139 - €419
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Description

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Antibody Name:Anti-FZD7 Antibody
Antibody SKU:CAB4213
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FZD7 (NP_003498.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:SW480, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human FZD7 (NP_003498.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRDP GAAA PLSS LGLC ALVL ALLG ALSA GAGA QPYH GEKG ISVP DHGF CQPI SIPL CTDI AYNQ TILP NLLG HTNQ EDAG LEVH QFYP LVKV QCSP ELRF FLCS MYAP VCTV LDQA IPPC RSLC ERAR QGCE ALMN KFGF QWPE RLRC ENFP VHGA GEIC VGQN TSDG SGGP GGGP TAYP TAPY LPDL PFTA LPPG ASDG RGRP AFPF SCPR QLKV PPYL GYRF LGER DCGA PCEP GRAN GLMY FKEE ERRF ARLW VGVW
Gene ID:8324
Uniprot:O75084
Cellular Location:Cell membrane, Membrane, Multi-pass membrane protein
Calculated MW:63kDa
Observed MW:70-72kDa
Synonyms:FZD7, FzE3
Background: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas.
UniProt Protein Function:FZD7: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.
UniProt Protein Details:

Protein type:Receptor, GPCR; Motility/polarity/chemotaxis; Membrane protein, multi-pass; GPCR, Fz/Smo family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to membrane; plasma membrane

Molecular Function:G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; frizzled binding; PDZ domain binding

Biological Process: neuron differentiation; G-protein coupled receptor protein signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of epithelial cell proliferation involved in wound healing; stem cell maintenance; T cell differentiation in the thymus; positive regulation of JNK cascade; somatic stem cell division; Wnt receptor signaling pathway through beta-catenin; satellite cell compartment self-renewal involved in skeletal muscle regeneration; negative regulation of ectodermal cell fate specification; positive regulation of phosphorylation

NCBI Summary:Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD7 protein contains an N-terminal signal sequence, 10 cysteine residues typical of the cysteine-rich extracellular domain of Fz family members, 7 putative transmembrane domains, and an intracellular C-terminal tail with a PDZ domain-binding motif. FZD7 gene expression may downregulate APC function and enhance beta-catenin-mediated signals in poorly differentiated human esophageal carcinomas. [provided by RefSeq, Jul 2008]
UniProt Code:O75084
NCBI GenInfo Identifier:52788226
NCBI Gene ID:8324
NCBI Accession:O75084.2
UniProt Related Accession:O75084
Molecular Weight:~ 64, 85kDa
NCBI Full Name:Frizzled-7
NCBI Synonym Full Names:frizzled class receptor 7
NCBI Official Symbol:FZD7  
NCBI Official Synonym Symbols:FzE3  
NCBI Protein Information:frizzled-7
UniProt Protein Name:Frizzled-7
UniProt Synonym Protein Names:FzE3
Protein Family:Frizzled
UniProt Gene Name:FZD7  
UniProt Entry Name:FZD7_HUMAN
Anti-FZD7 Antibody (CAB4213)Western blot analysis of extracts of various cell lines, using FZD7 antibody (CAB4213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

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