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Free Fatty Acids Assay Kit - Fluorometric (MAES0010)

SKU:
MAES0010
Product Type:
Assay
Detection Method:
Fluorometric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Animal Tissue
Sensitivity:
0.58 umol/L
Range:
0.58-20 umol/L
Assay Time:
70 min
Shipping:
Gel Pack
Research Area:
Diabetes & Obesity
Food Safety & Analysis
Clinical Chemistry
$731
Frequently bought together:

Description

system_update_altDatasheet
Product Name:Free Fatty Acids Assay Kit - Fluorometric
Product Code:MAES0010
Product Size:96 Assays
Sample Type:Serum, plasma, animal tissue
Assay Time:70 min
Instrument:Fluorescence Microplate Reader
Sensitivity:0.58 µmol/L
Detection Range:0.58-20 µmol/L
Recovery Rate:96
Inter CV:5.3
Inter CV:4.2

Free fatty acids, also known as non-esterified fatty acids, are derived from dietary or the metabolism of adipose tissue. In adipose tissue, hormone-sensitive lipase (HSL) decomposes triglycerides to produce glycerol and fatty acids. Circulating in the body with free fatty acids combined with plasma albumin, used as an energy source easily absorbed by muscles, brains, and other tissues and organs. FFA is not only the product of fat hydrolysis, but also the substrate of fat synthesis. The concentration of FFA is related to lipid metabolism, glucose metabolism and endocrine function.

Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen Free fatty acids produce acyl coenzyme A in the presence of acyl synthase, which produces hydrogen peroxide in the presence of acyl oxidase. In the presence of the enzyme and probe, hydrogen peroxide react to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is directly proportional to the concentration of free fatty acids.

Shelf Life:6 Months
Storage:-20°C
Shipping:Gel Pack
Research Area:Lipids metabolism

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