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Free Fatty Acid Measurement Kit (BA0101)

SKU:
BA0101
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Urine, Saliva, Milk, Cell Culture, Food
Research Area:
Lipid Metabolism
Food Safety & Analysis
Plant & Environmental Stress
Clinical Chemistry
€959
Frequently bought together:

Description

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Free Fatty Acid Measurement Kit

Fatty acidsare aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil and wax. Fatty Acids play important roles in cellular synthesis, energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome and Reye Syndrome.

Assay Genie's free fatty acid measurement kit provides a simple, one-step and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to HO. The resulting HO reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.

Applications

For quantitative determination of fatty acid and evaluation of drug effects on its metabolism.

Key Features

  • Sensitive. Use 10 uL samples. Linear detection range: colorimetric assay 7 - 1000 uM, fluorimetric assay 7 - 100 uM fatty acid.
  • Convenient. Room temperature "mix-and-read" procedure can be readily automated for high-throughput assay of thousands of samples per day.
New Column New Column
Kit Includes:
  • Assay Buffer: 20 mL
  • Dye Reagent: 120 mL
  • Enzyme A: Dried Enzyme B: 120 mL
  • CoSubstrate: 120 mL
  • Standard: 1 mL
  • 1 mM palmitic acid
Kit Requires:
Pipetting devices, centrifuge tubes, clear flat-bottom uncoated 96-well plates, optical density plate reader; black flat-bottom uncoated 96-well plates, fluorescence plate reader. For milk and solid samples, 0.45mm PTFE syringe filter and 5% isopropanol, 5% Triton X-100 solution.
Method of Detection:
OD570nm, or FL530/585nm
Detection Limit:
7 uM
Samples:
Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc
Species:
All
Protocol Length:
30 min
Size:
100 tests
Storage:
Store all components at -20°C
Shelf Life:
6 months

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