FQNs (Fluoroquinolones) ELISA Kit (FSES0037)

SKU:

FSES0037

Product Type:

ELISA Kit

Applications:

ELISA

Research Area:

Food Science

Description

Description

FQNs (Fluoroquinolones) ELISA Kit

Kit Name:

FQNs (Fluoroquinolones) ELISA Kit

Kit Size:

96 Assays

Test Principle:

This kit uses Competitive-ELISA as the method. It can detect Fluoroquinolones/Quinolones (FQNS) in samples, such as milk, muscle, honey, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate in this kit has been pre-coated with coupled antigen. During the reaction, FQNS in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-FQNS antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and substrate reagent is added for color development. There is a negative correlation between the OD value of samples and the concentration of FQNS. The concentration of FQNS in the samples can be calculated by comparing the OD of the samples to the standard curve.

Sensitivity:

0.2 ppb (ng/mL)

Reaction Mode:

25°C,45 min~15min

Detection Limit:

Muscle ---0.6 ppb; Honey ---0.8 ppb; Milk---6 ppb; Milk powder---12 ppb; Eggs---6 ppb; Urine---1 ppb.

Cross Reactivity:

Enrofloxacin	100%
Norfloxacin	174%
Ciprofloxacin	170%
Lomefloxacin	132%
Flumequine	126%
Peflacine	125%
Danofloxacin	110%
Sarafloxacin	107%
Difloxacin	84%
Enoxacin	66%
Ofloxacin (racemate)	58%
Oxolinic acid	28%
Levofloxacin	10%
Marbofloxacin	4%

Sample Recovery Rate:

Muscle, Honey, Milk, Milk powder, Eggs, Urine ---85%±15%.

Kits Components:

Enrofloxacin	100%
Norfloxacin	174%
Ciprofloxacin	170%
Lomefloxacin	132%
Flumequine	126%
Peflacine	125%
Danofloxacin	110%
Sarafloxacin	107%
Difloxacin	84%
Enoxacin	66%
Ofloxacin (racemate)	58%
Oxolinic acid	28%
Levofloxacin	10%
Marbofloxacin	4%

Instruments:

Microplate reader, Printer, Homogenizer, Oscillators, Nitrogen evaporators, Water bath, Centrifuge, Graduated pipette, Balance (sensibility 0.01g).

Micropipette:

Single channel (20-200 uL, 100-1000 uL), Multichannel (30-300 uL).

Reagents:

Anhydrous acetonitrile, N-hexane, Concentrated HCl, Dichloromethane

Storage And Expiry Date:

Store the kit at 2~8°C. Do not freeze any test kit components. Return any unused microwells to their original foil bag and reseal them together with the desiccant provided and further store at 2 - 8°C. Expiry date: expiration date is on the packing box.