The Anti-FPGS Antibody (CAB20973) is a polyclonal antibody specifically designed for research involving FPGS, a key enzyme involved in folate metabolism and the activation of antifolate drugs used in cancer chemotherapy. This antibody, raised in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the FPGS protein, this antibody allows for the detection and analysis of FPGS in various cell types, making it ideal for studies in cancer research and drug development.FPGS plays a crucial role in the intracellular activation of antifolate drugs, such as methotrexate, which are widely used in cancer treatment.
Understanding the activity and regulation of FPGS is essential for optimizing the efficacy of these drugs and developing new therapeutic strategies for cancer patients. The Anti-FPGS Antibody provides researchers with a valuable tool for investigating the role of FPGS in cancer biology and drug response, ultimately contributing to the development of more effective and personalized treatment approaches.
Product Name:
FPGS Monoclonal Antibody
SKU:
CAB20973
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 450-587 of human FPGS (Q05932).
This gene encodes the folylpolyglutamate synthetase enzyme. This enzyme has a central role in establishing and maintaining both cytosolic and mitochondrial folylpolyglutamate concentrations and, therefore, is essential for folate homeostasis and the survival of proliferating cells. This enzyme catalyzes the ATP-dependent addition of glutamate moieties to folate and folate derivatives. Alternative splicing results in transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
2356
Clone Number:
ARC2964
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using FPGS antibody (CAB20973) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
