The Foxp3 Rabbit Monoclonal Antibody (CAB4953) is a valuable tool for researchers studying immune regulation and T cell biology. Foxp3, also known as forkhead box P3, is a key transcription factor that plays a critical role in the development and function of regulatory T cells (Tregs). This antibody, generated in rabbits, is highly specific and reacts with human samples, making it suitable for a variety of research applications including immunohistochemistry, flow cytometry, and Western blotting.Tregs are a subset of T cells that play a crucial role in maintaining immune tolerance and preventing autoimmunity. Dysregulation of Tregs and Foxp3 expression has been implicated in a variety of autoimmune diseases, inflammatory conditions, and cancer.
The ability to accurately detect and quantify Foxp3 expression is essential for understanding the mechanisms underlying these diseases and developing targeted therapies.By using the Foxp3 Rabbit Monoclonal Antibody (CAB4953), researchers can investigate the role of Foxp3 in immune tolerance, inflammation, and disease progression. This antibody offers high sensitivity and specificity, allowing for precise detection of Foxp3 expression in various cell types and tissues. Overall, this antibody is a valuable tool for advancing our understanding of immune regulation and developing new treatments for a range of diseases.
Product Name:
FOXP3 Rabbit Monoclonal Antibody
SKU:
CAB4953
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 286-431 of human FOXP3 (Q9BZS1).
WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
JM2; AIID; IPEX; PIDX; XPID; DIETER; FOXP3
Positive Sample:
Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
50943
Clone Number:
ARC1159
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using FOXP3 Rabbit mAb (CAB4953) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
