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FOXO1 (Phospho-Ser319) Transcription Factor Activity Assay

SKU:
TFAB00027
Product Type:
ELISA Kit
ELISA Type:
Transcription Factor Assay
Analytes:
FOXO1 (Phospho-Ser319)
Reactivity:
Human
Mouse
Rat
$719
Frequently bought together:

Description

system_update_altDatasheet

FOXO1 (Phospho-Ser319)Transcription Factor Activity Assay

The Foxo1 Phospho-Ser319 Transcription Factor Activity Assay Kit is designed for the precise measurement of Foxo1 phosphorylation at serine 319 in various samples such as cell lysates and tissue homogenates. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for research purposes.Foxo1 is a key transcription factor involved in various cellular processes, including cell cycle regulation, apoptosis, and metabolism. Phosphorylation of Foxo1 at serine 319 can modulate its transcriptional activity, impacting gene expression related to insulin sensitivity, oxidative stress, and longevity.

This assay kit provides researchers with a valuable tool for studying the role of Foxo1 phosphorylation in various physiological and pathological conditions, including diabetes, aging, and cancer. By accurately measuring Foxo1 activity, researchers can gain insight into the molecular mechanisms underlying these diseases and potentially identify new therapeutic targets.

Product Name:FOXO1 (Phospho-Ser319) Transcription Factor Activity Assay
Product Code:TFAB00027
Target:FOXO1 (Phospho-Ser319)
Synonyms:Forkhead box protein O1A, Forkhead in rhabdomyosarcoma
Reactivity:Human, Mouse, Rat
Sample Types:Nuclear or cell lysates

The Assay Genie FOXO1 (Phospho-Ser319) transcription factor activity assay allows for the detection and qualitative analysis of FOXO1 (Phospho-Ser319) active transcription factors in eukaryotic nuclear or cell lysates.

Assay Genie ELISA kits are designed to significantly reduce experiment time and ensure sensitivity and flexibility for high-throughput screening.

Assay Time:4.5 hours
Detection Method:Colorimetric 450 nm
Size:12 x 8-Well Microstrips
Storage:4°C for 6 months
UniProt Protein Function:FOXO1A: a transcription factor of the forkhead family. A central regulator of metabolism in several cell types. May play an important role in myogenic growth and differentiation, and in the regulation of lipolysis in adipocytes by controlling the expression of adipose triglyceride lipase (ATGL), the rate-limiting lipolytic enzyme. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. Contains 1 fork-head domain.
UniProt Protein Details:

Protein type:Nuclear receptor co-regulator; Oncoprotein; DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 13q14.1

Cellular Component: cytoplasm; cytosol; mitochondrion; nucleoplasm; nucleus

Molecular Function:beta-catenin binding; chromatin binding; protein binding; protein phosphatase 2A binding; sequence-specific DNA binding; ubiquitin protein ligase binding

Biological Process: anatomical structure morphogenesis; apoptosis; autophagy; blood vessel development; cell differentiation; cell glucose homeostasis; cellular response to insulin stimulus; cellular response to starvation; endocrine pancreas development; epidermal growth factor receptor signaling pathway; fat cell differentiation; fibroblast growth factor receptor signaling pathway; innate immune response; insulin receptor signaling pathway; negative regulation of apoptosis; negative regulation of fat cell differentiation; negative regulation of stress-activated MAPK cascade; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of apoptosis; positive regulation of autophagy; positive regulation of gluconeogenesis; positive regulation of protein catabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein amino acid acetylation; response to DNA damage stimulus; thermoregulation; transcription, DNA-dependent

Disease: Rhabdomyosarcoma 2

NCBI Summary:This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
UniProt Code:Q12778
NCBI GenInfo Identifier:116241368
NCBI Gene ID:2308
NCBI Accession:Q12778.2
UniProt Secondary Accession:Q12778,O43523, Q5VYC7, Q6NSK6,
UniProt Related Accession:Q12778
Molecular Weight:
NCBI Full Name:Forkhead box protein O1
NCBI Synonym Full Names:forkhead box O1
NCBI Official Symbol:FOXO1  
NCBI Official Synonym Symbols:FKH1; FKHR; FOXO1A  
NCBI Protein Information:forkhead box protein O1
UniProt Protein Name:Forkhead box protein O1
UniProt Synonym Protein Names:Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
Protein Family:Foxo1-corepressor
UniProt Gene Name:FOXO1  
UniProt Entry Name:FOXO1_HUMAN
AntibodiesELISA Kits
Anti-FOXO1 Antibody (CAB13862)Human FOXO1 (Forkhead Box Protein O1) ELISA Kit (HUES02193)
Anti-Acetyl-FOXO1-K262/K265/K274 Antibody (CAB17406)FOXO1 (Phospho-Ser256) Transcription Factor Activity Assay
Anti-FOXO1 Antibody (CAB2934)FOXO1 Transcription Factor Activity Assay
Anti-Phospho-FoxO1-S256 Antibody (CABP0172)FOXO1A Transcription Factor Activity Assay
Anti-Phospho-FOXO1-S319 Antibody (CABP0176)

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