The FOXN4 Polyclonal Antibody (PACO22335) is a valuable tool for researchers studying the FOXN4 protein, a transcription factor known for its role in regulating gene expression in the development and maintenance of the thymus gland. This antibody, raised in rabbits, is highly specific for detecting FOXN4 in human samples and has been validated for use in Western blot applications. By binding to the FOXN4 protein, this antibody enables researchers to analyze its expression levels in various cell types, making it ideal for studies in developmental biology and immunology.
FOXN4 is crucial for the differentiation of thymic epithelial cells and the production of T cells, key players in the immune response. Dysregulation of FOXN4 expression has been implicated in autoimmune disorders and thymic abnormalities, highlighting the importance of understanding its function in normal and diseased states. By studying the activity of FOXN4, researchers can gain insight into thymus development and immune system function, paving the way for potential therapeutic strategies targeting FOXN4-related diseases.
Antibody Name:
FOXN4 Antibody (PACO22335)
Antibody SKU:
PACO22335
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human FOXN4.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from LOVO cells, using FOXN4 antibody.
Background:
Transcription factor essential for neural and some non-neural tissues development, such as retina and lung respectively. Binds to an 11-bp consensus sequence containing the invariant tetranucleotide 5'-ACGC-3'. During development of the central nervous system, is required to specify the amacrine and horizontal cell fates from multipotent retinal progenitors while suppressing the alternative photoreceptor cell fates through activating DLL4-NOTCH signaling. Also acts synergistically with ASCL1/MASH1 to activate DLL4-NOTCH signaling and drive commitment of p2 progenitors to the V2b interneuron fates during spinal cord neurogenesis. In development of non-neural tissues, plays an essential role in the specification of the atrioventricular canal and is indirectly required for patterning the distal airway during lung development By similarity.
Synonyms:
Forkhead box protein N4; FOXN4;
UniProt Protein Function:
FOXN4: 2 isoforms of the human protein are produced by alternative splicing.Protein type: Cell development/differentiation; DNA-binding; Transcription factorChromosomal Location of Human Ortholog: 12q24.11Cellular Component: nucleusMolecular Function: chromatin binding; transcription factor activityBiological Process: heart looping; positive regulation of transcription, DNA-dependent; regulation of heart contraction; regulation of transcription, DNA-dependent
UniProt Protein Details:
NCBI Summary:
Members of the winged-helix/forkhead family of transcription factors, such as FOXN4, are characterized by a 110-amino acid DNA-binding domain that can fold into a variant of the helix-turn-helix motif consisting of 3 alpha helices flanked by 2 large loops or wings. These transcription factors are involved in a variety of biologic processes as key regulators in development and metabolism (Li et al., 2004 [PubMed 15363391]).[supplied by OMIM, Mar 2008]
