The FOXJ1 Polyclonal Antibody is a vital tool for researchers studying the FOXJ1 protein, a transcription factor essential for ciliogenesis and motile cilia formation. This antibody, generated in rabbits, demonstrates high reactivity with human samples and has been validated for use in various applications, including Western blotting. By specifically binding to the FOXJ1 protein, this antibody allows for the accurate detection and analysis of FOXJ1 expression in a wide range of cell types.FOXJ1 is a key regulator of cilia development and function, playing a crucial role in processes such as mucociliary clearance in the respiratory tract and left-right axis establishment during embryonic development.
Dysregulation of FOXJ1 has been linked to various diseases, including respiratory disorders and ciliopathies. Therefore, research into FOXJ1 function is essential for understanding the molecular mechanisms underlying these conditions and developing potential therapeutic interventions.By utilizing the FOXJ1 Polyclonal Antibody, researchers can delve deeper into the mechanisms governing ciliogenesis and cilia-driven processes, advancing our understanding of the role of FOXJ1 in health and disease. This antibody is a valuable tool for investigations in areas such as developmental biology, respiratory biology, and genetic disorders associated with cilia dysfunction.
WB,1:1000 - 1:5000 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
HFH4; HFH-4; CILD43; FKHL13; FOXJ1
Positive Sample:
Mouse brain,
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Secreted, Single-pass type I membrane protein.
Calculated MW:
45kDa
Observed MW:
55kDa/
This gene encodes a member of the forkhead family of transcription factors. Similar genes in zebrafish and mouse have been shown to regulate the transcription of genes that control the production of motile cilia. The mouse ortholog also functions in the determination of left-right asymmetry. Polymorphisms in this gene are associated with systemic lupus erythematosus and allergic rhinitis.
Purification Method:
Affinity purification
Gene ID:
2302
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain using FOXJ1 Rabbit pAb(CAB23024) at 1:3000 dilution.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection:ECL Basic Kit (AbGn00020).Exposuretime:10s.
